Pteisolic acid G, a novel ent‑kaurane diterpenoid, inhibits viability and induces apoptosis in human colorectal carcinoma cells

摘要: Human colorectal cancer (CRC) is a major cause of morbidity and mortality, its incidence rates are increasing in economical transitioning areas globally. To develop efficient chemotherapy drugs for CRC, the present study isolated identified novel ent-kaurane diterpenoid from Pteris semipinnata, termed pterisolic acid G (PAG). This was demonstrated to significantly inhibit growth human CRC HCT116 cells time- dose-dependent manner, determined using Cell Counting Kit-8 assay. Additionally, western blot analysis, Hoechst 33342 staining cytometry analysis revealed that PAG not only inhibited viability by suppressing dishevelled segment polarity protein 2/glycogen synthase kinase 3 β/β-catenin pathway, but also induced apoptosis downregulating nuclear factor-κB p65 activity, stimulating p53 expression promoting generation intracellular reactive oxygen species. These results suggest PAG, inhibitor Wnt/β-catenin pathway inducer apoptosis, should be investigated more detail vivo experiments comprehensive mechanistic studies order examine potential use as therapeutic agent treatment CRC.