Comparison of 15N2 and acetylene reduction methods for the measurement of nitrogen fixation by Baltic Sea cyanobacteria

作者: P. Moisander , J. Lehtimäki , K. Sivonen , K. Kononen

DOI: 10.2216/I0031-8884-35-6S-140.1

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摘要: Abstract The aim of this study was to compare nitrogen fixation rates by cyanobacteria measured acetylene reduction and 15N2, fixation. effect environmental stress on the theoretical conversion factor 4 (mol C2H2 per mol N2 reduced) between methods studied experimentally in cultures field samples from open Baltic Sea. Axenic non-axenic laboratory Nodularia spumigena Mertens (strain BY1). sphaerocarpa Bornet et Flahault HKVV) Aphanizomenon cf. flos-aquae Ralfs ex TR183) isolated Sea were used test salinity NH4+ concentration. 0.6–4.8 for spumigena. 2.6–11.0 Nodulorio 1.0–15.5 Aphanizomenon. Changes concentrations did not affect factors. but varied among cyanobacterial strains growth phases. sample concentration light level conversio...

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