Identification of Torque Teno Virus in Culture-Negative Endophthalmitis by Representational Deep DNA Sequencing
作者： Aaron Y. Lee , Lakshmi Akileswaran , Michael D. Tibbetts , Sunir J. Garg , Russell N. Van Gelder
DOI: 10.1016/J.OPHTHA.2014.09.001
关键词:
摘要: Purpose To test the hypothesis that uncultured organisms may be present in cases of culture-negative endophthalmitis by use deep DNA sequencing vitreous biopsies. Design Single-center, consecutive, prospective, observational study. Participants Aqueous or biopsies from 21 consecutive patients presenting with presumed infectious and 7 samples undergoing surgery for noninfectious retinal disorders. Methods Traditional bacterial fungal culture, 16S quantitative polymerase chain reaction (qPCR), a representational deep-sequencing method (biome silico karyotyping [BRiSK]) were applied parallel to identify sequences corresponding potential pathogens. Main Outcome Measures Presence pathogen ocular samples. Results Zero control eyes routine yielded positive results bacteria virus culture (PCR). A total 14 (66.7%) harboring suspected culture-positive, most common being Staphylococcal Streptococcal species. There was good agreement among PCR, BRiSK methodologies culture-positive (Fleiss' kappa 0.621). PCR did not yield recognizable sequence any sample, whereas suggested presence Streptococcus 1 sample. With BRiSK, 57.1% 100% demonstrated torque teno (TTV) sequences, compared none controls ( P = 0.0005, Fisher exact test). The TTV viral confirmed qPCR. No other known viruses pathogens identified these Conclusions Culture, qPCR, provide complementary information endophthalmitis. majority contain significant levels DNA. "Culture negativity" does seem due failure growth fastidious bacteria. small unexpectedly found all some This study cannot distinguish whether is direct intraocular pathogen, an adjuvant inflammation, general marker commensal but provides testable pathogenic mechanism
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