Monoclonal Antibodies against the Minimal DNA-Binding Domain in the Carboxyl-Terminal Region of Human Immunodeficiency Virus Type 1 Integrase
作者： Tetsuya Ishikawa , Nobuo Okui , Noriko Kobayashi , Ryuta Sakuma , Tadaichi Kitamura
DOI: 10.1128/JVI.73.5.4475-4480.1999
关键词:
摘要: Integrase of human immunodeficiency virus type 1 (HIVIN) consists 288 amino acids, and its minimum DNA-binding domain (MDBD) (amino acids [aa] 220 to 270) is required for the integration reaction. We produced characterized four murine monoclonal antibodies (MAbs) MDBD HIVIN (strain LAI). Immunoblot enzyme-linked immunosorbent assays with truncated HIVINs showed that those MAbs recognized sequential epitopes within (aa 228 236, 237 252, 253 261, 262 270). Their binding inhibited terminal cleavage strand transfer activities but not disintegration activity in vitro. This collection useful studying structure function by complementing mutational analyses other biochemical studies.
sci-hub.st 参考文章(41)
DIANE BIZUB-BENDER, JOSEPH KULKOSKY, ANNA MARIE SKALKA, Monoclonal Antibodies against HIV Type 1 Integrase: Clues to Molecular Structure AIDS Research and Human Retroviruses. ,vol. 10, pp. 1105- 1115 ,(1994) , 10.1089/AID.1994.10.1105
Amy M. Woerner, Carol J. Marcus-Sekura, Characterization of a DNA binding domain in the C-terminus of HIV-1 integrase by deletion mutagenesis Nucleic Acids Research. ,vol. 21, pp. 3507- 3511 ,(1993) , 10.1093/NAR/21.15.3507
Tamio Fujiwara, Kiyoshi Mizuuchi, Retroviral DNA integration: Structure of an integration intermediate Cell. ,vol. 54, pp. 497- 504 ,(1988) , 10.1016/0092-8674(88)90071-2
Ramon A. Puras Lutzke, Cornells Vink, Ronald H.A. Plasterk, Characterization of the minimal DNA-binding domain of the HIV integrase protein Nucleic Acids Research. ,vol. 22, pp. 4125- 4131 ,(1994) , 10.1093/NAR/22.20.4125
S. Chow, K. Vincent, V Ellison, P. Brown, Reversal of integration and DNA splicing mediated by integrase of human immunodeficiency virus. Science. ,vol. 255, pp. 723- 726 ,(1992) , 10.1126/SCIENCE.1738845
Michael Schauer, Andreas Billich, The N-terminal region of HIV-1 integrase is required for integration activity, but not for DNA-binding Biochemical and Biophysical Research Communications. ,vol. 185, pp. 874- 880 ,(1992) , 10.1016/0006-291X(92)91708-X
Meera Drelich, Roland Wilhelm, Jan Mous, Identification of amino acid residues critical for endonuclease and integration activities of HIV-1 IN protein in vitro. Virology. ,vol. 188, pp. 459- 468 ,(1992) , 10.1016/0042-6822(92)90499-F
F. D. Bushman, A. Engelman, I. Palmer, P. Wingfield, R. Craigie, Domains of the integrase protein of human immunodeficiency virus type 1 responsible for polynucleotidyl transfer and zinc binding. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 90, pp. 3428- 3432 ,(1993) , 10.1073/PNAS.90.8.3428
Astrid P.A.M. Eijkelenboom, Ramon A. Puras Lutzke, Rolf Boelens, Ronald H.A. Plasterk, Robert Kaptein, Karl Hård, The DNA-binding domain of HIV-1 integrase has an SH3-like fold. Nature Structural & Molecular Biology. ,vol. 2, pp. 807- 810 ,(1995) , 10.1038/NSB0995-807
Lee Ratner, William Haseltine, Roberto Patarca, Kenneth J. Livak, Bruno Starcich, Steven F. Josephs, Ellen R. Doran, J. Antoni Rafalski, Erik A. Whitehorn, Kirk Baumeister, Lucinda Ivanoff, Stephen R. Petteway, Mark L. Pearson, James A. Lautenberger, Takis S. Papas, John Ghrayeb, Nancy T. Chang, Robert C. Gallo, Flossie Wong-Staal, Complete nucleotide sequence of the AIDS virus, HTLV-III Nature. ,vol. 313, pp. 277- 284 ,(1985) , 10.1038/313277A0