Nuclear DNA-binding proteins.
作者： Kristen L. Kucharczuk , David J. Goldhamer
DOI: 10.1016/S0091-679X(08)60391-X
关键词:
摘要: Publisher Summary This chapter discusses and provides protocols for electromobility shift assays (EMSAs) DNase I protection assays, presents particular experiments that exemplify important biological paradigms or methodologies in muscle research. A method nuclear extract preparation is discussed. The examples given this demonstrate the EMSA are powerful tools identification study of DNA-binding proteins involved myogenesis. When used conjunction with extracts, these techniques can map muscle-specific promoters enhancers, determine precise DNA sequence requirements protein binding, yield information concerning developmental stage tissue specificity activities, facilitate cloning novel factors. Once have been cloned, same be to characterize both sequences which they bind. interpreted light corroborating functional studies, EMSAs provide a strong foundation understanding transcriptional mechanisms, driving differentiation determination.
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