Presence, distribution, and diversity of iron-oxidizing bacteria at a landfill leachate-impacted groundwater surface water interface.

作者: Ping Gan , Allison A. MacKay , Shouliang Zhang , Barth F. Smets

DOI: 10.1111/J.1574-6941.2009.00797.X

关键词:

摘要: We examined the presence of iron-oxidizing bacteria (IOB) at a groundwater surface water interface (GSI) impacted by reduced originating as leachate from an upgradient landfill. IOB enrichments and quantifications were obtained, high vertical resolution, iron/oxygen opposing gradient cultivation method. The depth-resolved soil distribution profiles content, Fe(2+), total Fe indicated sharp gradients within top 10 cm sediments GSI, where density was highest. In addition, iron-reducing same sampling site mirrored distribution. Clone libraries two separate stratified community with clear differences short distances. Alpha- Betaproteobacteria dominant phylotypes. Clones near-surface sediment (1-2 below ground surface) dominated members Bradyrhizobiaceae Comamonadaceae; clones deeper phylogenetically more diverse, Rhodocyclaceae. iron deposition that active oxidation occurred only near-to-surface GSI sediments. match between abundance strongly hints contribution to in this Fe-rich ecosystem.

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