The 2,4-dinitrophenyl group for protection of hydroxyl function of tyrosine during solid-phase peptide synthesis.
作者： RACHEL PHILOSOF-OPPENHEIMER , ISRAEL PECHT , MATI FRIDKIN
DOI: 10.1111/J.1399-3011.1995.TB01029.X
关键词:
摘要: The facile thiolytic cleavage of the O-2,4-dinitrophenyl (Dnp) tyrosine bond was applied to solid-phase synthesis 22-amino acid residue peptide H-Asp-Ala-Val-Tyr-Thr-Gly-Leu-Asn-Thr-Arg-Asn-Gln-Glu-Thr-Tyr-Glu-Thr-Leu-Lys-His-Glu-Lys-OH, corresponding positions 62-83 in chain type 1 receptor for Fce, domains expressed on rat mucosal-type mast cells (line RBL-2H3). A method spectrophotometric determination insoluble O-Dnp as well unprotected phenolic moieties developed. It is based monitoring S-Dnp-2-mercaptoethanol, produced upon thiolysis by 2-mercaptoethanol. © Munksgaard 1995. Dedicated memory Dr. Susumu Funakoshi, a dear friend and leader chemistry.
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