Actomyosin dynamics, Bmp and Notch signaling pathways drive apical extrusion of proepicardial cells
作者： Laura Andrés-Delgado , Alexander Ernst , María Galardi-Castilla , David Bazaga , Marina Peralta
DOI: 10.1101/332593
关键词:
摘要: The epicardium, the outer mesothelial layer enclosing myocardium, plays key roles in heart development and regeneration. During embryogenesis it arises from proepicardium (PE), a cell cluster that appears dorsal pericardium close to venous pole of heart. Little is known about how PE emerges pericardial mesothelium. Using zebrafish model combination genetic tools, pharmacological agents quantitative vivo imaging we reveal coordinated collective movement drives formation. We found cells are apically extruded response actomyosin activity. Our results action Notch/Bmp pathways critically needed for apical extrusion cells. More generally, by comparison elimination unfit epithelia, our describe unique mechanism where viability maintained.
sci-hub.st 参考文章(100)
Andreas Holzinger, Jasplakinolide. An actin-specific reagent that promotes actin polymerization. Methods of Molecular Biology. ,vol. 161, pp. 71- 87 ,(2009) , 10.1007/978-1-60761-376-3_4
E. Michael Ostap, 2,3-Butanedione monoxime (BDM) as a myosin inhibitor. Journal of Muscle Research and Cell Motility. ,vol. 23, pp. 305- 308 ,(2002) , 10.1023/A:1022047102064
Christian Mosimann, Leonard I. Zon, Advanced zebrafish transgenesis with Tol2 and application for Cre/lox recombination experiments. Methods in Cell Biology. ,vol. 104, pp. 173- 194 ,(2011) , 10.1016/B978-0-12-374814-0.00010-0
Taro QP Noguchi, Masatoshi Morimatsu, Atsuko H Iwane, Toshio Yanagida, Taro QP Uyeda, None, The Role of Structural Dynamics of Actin in Class-Specific Myosin Motility PLOS ONE. ,vol. 10, pp. e0126262- ,(2015) , 10.1371/JOURNAL.PONE.0126262
Fay Wang, John Flanagan, Nan Su, Li-Chong Wang, Son Bui, Allissa Nielson, Xingyong Wu, Hong-Thuy Vo, Xiao-Jun Ma, Yuling Luo, RNAscope: A Novel in Situ RNA Analysis Platform for Formalin-Fixed, Paraffin-Embedded Tissues The Journal of Molecular Diagnostics. ,vol. 14, pp. 22- 29 ,(2012) , 10.1016/J.JMOLDX.2011.08.002
Matthew C Gibson, Norbert Perrimon, Extrusion and death of DPP/BMP-compromised epithelial cells in the developing Drosophila wing. Science. ,vol. 307, pp. 1785- 1789 ,(2005) , 10.1126/SCIENCE.1104751
Alec D Witty, Anton Mihic, Roger Y Tam, Stephanie A Fisher, Alexander Mikryukov, Molly S Shoichet, Ren-Ke Li, Steven J Kattman, Gordon Keller, Generation of the epicardial lineage from human pluripotent stem cells Nature Biotechnology. ,vol. 32, pp. 1026- 1035 ,(2014) , 10.1038/NBT.3002
K. Asakawa, M. L. Suster, K. Mizusawa, S. Nagayoshi, T. Kotani, A. Urasaki, Y. Kishimoto, M. Hibi, K. Kawakami, Genetic dissection of neural circuits by Tol2 transposon-mediated Gal4 gene and enhancer trapping in zebrafish Proceedings of the National Academy of Sciences of the United States of America. ,vol. 105, pp. 1255- 1260 ,(2008) , 10.1073/PNAS.0704963105
Jody Rosenblatt, Martin C. Raff, Louise P. Cramer, An epithelial cell destined for apoptosis signals its neighbors to extrude it by an actin- and myosin-dependent mechanism Current Biology. ,vol. 11, pp. 1847- 1857 ,(2001) , 10.1016/S0960-9822(01)00587-5
Thomas Lecuit, Alpha S. Yap, E-cadherin junctions as active mechanical integrators in tissue dynamics Nature Cell Biology. ,vol. 17, pp. 533- 539 ,(2015) , 10.1038/NCB3136