Rolling circle replication of padlock probes
作者： Ulf Landegren
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摘要: Rolling circle replication of a padlock primer is inhibited when it hybridised to target nucleic acid that long or circular. The invention provides methods addressing this problem including cutting the near preferably at site which hybridises with probe, whereby 3′-end cut acts as for rolling probe. Also included method assaying polyepitopic by use two affinity probes each carrying an oligonucleotide tag and probe in association probes.
google.nl 参考文章(9)
Jerry L Ruth, David A. Driver, Circular extension for generating multiple nucleic acid complements ,(1991)
Margaret Brandwein, David Y. Zhang, Nucleic acid amplification method: hybridization signal amplification method (hsam) ,(1997)
T Sano, C. Smith, C. Cantor, Immuno-PCR: very sensitive antigen detection by means of specific antibody-DNA conjugates. Science. ,vol. 258, pp. 120- 122 ,(1992) , 10.1126/SCIENCE.1439758
J. Baner, M. Nilsson, M. Mendel-Hartvig, U. Landegren, Signal amplification of padlock probes by rolling circle replication Nucleic Acids Research. ,vol. 26, pp. 5073- 5078 ,(1998) , 10.1093/NAR/26.22.5073
Margaret Brandwein, Terence C. H. Hsuih, David Y. Zhang, Nucleic acid amplification method: ramification-extension amplification method (RAM) ,(1997)
R D Joerger, T M Truby, E R Hendrickson, R M Young, R C Ebersole, Analyte detection with DNA-labeled antibodies and polymerase chain reaction Clinical Chemistry. ,vol. 41, pp. 1371- 1377 ,(1995) , 10.1093/CLINCHEM/41.9.1371
Paul M. Lizardi, Rolling circle replication reporter systems ,(1998)
Paul M. Lizardi, Michael Caplan, Unimolecular segment amplification and detection ,(1996)
Ulf Landegren, Immunoassay and kit with two reagents that are cross-linked if they adhere to an analyte ,(1996)