Comparison of some packings for reversed-phase high-performance liquid—solid chromatography

摘要: Summary A comparison is made between non-polar chemically bonded phases (CBP), pyrocarbon-modified silica gel (PMS) and carbon black (PMCB). The retention mechanism probably an adsorption process in all instances but the contribution of solute-solvent interactions to larger on CBP than PMS or PMCB, resulting smaller retentions a greater selectivity for polar compounds. Retentions homologous series show that group better (or PMCB) CBP. geometrical isomers also PMS. However, if important differences polarities appear, can be very selective phases. Changing water content solvent mixture generally does not provide variations selectivity. Such are achieved by changing modifier more loadability columns difficult when single compounds injected. If separation problems dealt with, most parameter chromatographic system. choice best packing therefore depends great extent separations question.