Effects of Cold Ischemia on Gene Expression: A Review and Commentary

作者: William E. Grizzle , Dennis Otali , Katherine C. Sexton , Daniel S. Atherton

DOI: 10.1089/BIO.2016.0013

关键词:

摘要: Frequently investigators request that tissues be collected and processed in less than one hour following removal from a patient. Some biorepositories expend significant personnel time other resources trying to meet such goals; however, it is unclear whether the perceived benefits of relatively short cold ischemia times warrant these added costs. The literature human surgical prospectively exposed at several points was reviewed compare changes transcripts/genes microRNA with ischemia. Also, reports protein response were correlated genes. limited; for most tissues, only small proportion (<1%) up 3 hours surgery transcripts increase rather decrease 2 Biorepositories must consider evidence molecular results tissu...

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