Effects of Selenium on Morphological Changes in Candida utilis ATCC 9950 Yeast Cells.
作者： Marek Kieliszek , Stanisław Błażejak , Anna Bzducha-Wróbel , Agnieszka Kurcz
DOI: 10.1007/S12011-015-0415-3
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摘要: This paper presents the results of microscopic examinations yeast cells cultured in extract–peptone–dextrose (YPD) media supplemented with sodium selenite(IV). The analysis morphological changes aimed to determine whether selected selenium doses and culturing time may affect this element accumulation cell structures a form inorganic or organic compounds, as result detoxification processes. range characteristic yeasts cultivated experimental selenite(IV) was observed, including shrinkage cytoplasm thickening within vacuole structure. processes disintegration were observed aging medium, which indicate presence so-called ghost lacking intracellular organelles occurring morphology selenite typical for stationary phase growth. From detailed observations, larger surface area (6.03 μm2) (2.17 μm2) noticed after 24-h medium 20 mg Se4+/L. coefficient shape enriched well control YPD ranged from 1.02 1.22. Elongation cultivation (up 48 72 h) caused reduction due
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