Ru(II)-Thymine Complex Causes Cell Growth Inhibition and Induction of Caspase-Mediated Apoptosis in Human Promyelocytic Leukemia HL-60 Cells.
作者： Maiara de Souza Oliveira , Ádila de Santana , Rodrigo Correa , Milena Soares , Alzir Batista
DOI: 10.3390/IJMS19061609
关键词:
摘要: Ruthenium-based compounds represent a class of potential antineoplastic drugs. Recently, we designed, synthesized, and identified the Ru(II)-thymine complex [Ru(PPh3)2(Thy)(bipy)]PF6 (where PPh = triphenylphosphine, Thy thymine bipy 2,2′-bipyridine) as potent cytotoxic agent with ability to bind DNA human bovine serum albumins. In this study, underlying mechanism was assessed. This displayed cytotoxicity in different cancer cell lines; morphology that is associated apoptotic death, increased internucleosomal fragmentation without membrane permeability, loss mitochondrial transmembrane potential, phosphatidylserine externalization, caspase-3 activation were observed promyelocytic leukemia HL-60 cells treated complex. Moreover, pretreatment Z-VAD(OMe)-FMK, pan-caspase inhibitor, partially reduced apoptosis induced by complex, indicating death occurred through caspase-mediated pathway. conclusion, displays induces cells.
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