Bioprinting 101: Design, Fabrication, and Evaluation of Cell-Laden 3D Bioprinted Scaffolds.

摘要: 3D bioprinting is an additive manufacturing technique that recapitulates the native architecture of tissues. This accomplished through precise deposition cell-containing bioinks. The spatiotemporal control over bioink permits for improved communication between cells and extracellular matrix, facilitates fabrication anatomically physiologically relevant structures. physiochemical properties bioinks, before after crosslinking, are crucial complex tissue Specifically, rheological bioinks determines printability, structural fidelity, cell viability during printing process, whereas postcrosslinking critical their mechanical integrity, physiological stability, survival, functions. In this review, we critically evaluate design criteria, specifically extrusion-based techniques, to fabricate constructs. effects various processing parameters on biophysical biochemical characteristics discussed. Furthermore, emerging trends future directions in area also highlighted. Graphical abstract [Figure: see text] Impact statement Extrusion-based approach fabricating cell-laden engineered review evaluates criteria pre- post-printing evaluation approaches described, as well new research field development functional