Cyclic GMP-Dependent Protein Kinase Activation and Induction by Exisulind and CP461 in Colon Tumor Cells
作者： Rifat Pamukcu , Tashandra Underwood , Gerhard Sperl , Han Li , Mary David
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摘要: These studies report on the activation and induction of cGMP-dependent protein kinase (PKG) by exisulind analogs test hypothesis that PKG is involved in apoptosis colon tumor cells. Exisulind are proapoptotic drugs developed as inhibitors cGMP phosphodiesterase gene families 5 2 have been shown to sustain increased SW480 HT29 At concentrations induced apoptosis, both CP461 activity cell supernatants. was dose-dependent sustained. Activation also seen lines HT29, T84, HCT116. The guanylyl cyclase activators YC-1 guanylin secondary cellular had no direct effect purified or basal stimulated from An additional after 8 h drug treatment a increase Iβ expression. β-Catenin, potential new substrate for PKG, whose regulation influences phosphorylated vitro . 32P-labeled cells treated with showed phosphorylation β-catenin. data indicate activate induce resulting β-catenin enhanced promote death.
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