Transcriptome analysis identifies genes involved in ethanol response of Saccharomyces cerevisiae in Agave tequilana juice.

摘要: During ethanol fermentation, yeast cells are exposed to stress due the accumulation of ethanol, cell growth is altered and output target product reduced. For Agave beverages, like tequila, no reports have been published on global gene expression under stress. In this work, we used microarray analysis identify Saccharomyces cerevisiae genes involved in response. Gene a tequila strain S. (AR5) was explored by comparing with that laboratory S288C, both after exposure. Additionally, two different culture conditions, grown tequilana juice as natural fermentation media or yeast-extract peptone dextrose artificial media. Of 6368 microarray, 657 were identified had responses and/or A cluster 28 found over-expressed specifically AR5 could be adaptation 14 which unknown such yor343c, ylr162w, ygr182c, ymr265c, yer053c-a ydr415c. These most suitable for transforming increase tolerance process. Other response (RFC4, TSA1, MLH1, PAU3, RAD53) transport (CYB2, TIP20, QCR9) expressed same cluster. Unknown good candidates development recombinant yeasts use industrial fermentation.