Genomic Organization and Mutation Analysis ofp73in Oligodendrogliomas with Chromosome 1 p-Arm Deletions

摘要: p73, a protein having substantial structural and functional similarity to p53, has recently been identified demonstrated be potential tumor suppressor. Its location on human chromosome 1p36.33 implicates p73 as candidate for neuroblastoma. Like neuroblastoma, oligodendrogliomas also show high frequency of deletions in 1p36.3. To determine whether is suppressor gene involved the development oligodendrogliomas, we performed mutation analysis with 1 p-arm deletions. We first determined genomic organization intron-exon boundary sequences by long PCR, vectorette Southern hybridization. This spans about 65 kb large intron. Primer pairs amplification each 13 encoding exons were designed corresponding introns. The amplicons then analyzed using denaturing high-performance liquid chromatography system mutations gene. Twenty oligodendroglioma samples 1p36.3 screened, but no detected except several polymorphisms. It thus clear that not despite its frequently deleted region.