Biocatalytic preparation of acylated derivatives of flavonoid glycosides enhances their antioxidant and antimicrobial activity.

摘要: Enzymatic synthesis of acylated derivatives a monosaccharidic flavonoid chrysoeriol-7-O-beta-D-(3''-E-p-coumaroyl)-glucopyranoside as well disaccharidic chrysoeriol-7-[6'''-O-acetyl-beta-D-allosyl-(1-->2)-beta-D-glucopyranoside], isolated from Greek endemic plants, was performed using an immobilized Candida antarctica lipase in non-toxic organic solvents. The influence the reaction parameters such molar ratio acyl donor to flavonoid, nature donor, on performance biocatalytic process pointed out acylation naringin model reaction. With vinyl laurate highest conversion observed at relatively high (>or=10), acetone solvent. Lipase exhibits specificity towards primary alcohol glucose moiety both glycosides. introduction group into glucosylated flavonoids significantly improved their antioxidant activity LDL and serum vitro. Furthermore, derivative increased its antimicrobial against two Gram-positive bacteria.