Nerve growth factor prevents apoptosis of rat peritoneal mast cells through the trk proto-oncogene receptor
作者： Keiko Kawamoto , Toshiya Okada , Yukiko Kannan , Hiroko Ushio , Masahiro Matsumoto
DOI: 10.1182/BLOOD.V86.12.4638.BLOODJOURNAL86124638
关键词:
摘要: We investigated the inhibitory activity of nerve growth factor (NGF) on apoptosis rat peritoneal mast cells (PMCs) and compared it with that recombinant stem cell (rSCF), which is a factor. When PMCs were incubated up to 72 hours in presence control medium, internucleosomal fragmentation DNA indicating was detected by agarose gel electrophoresis flow cytometry. The aged showed morphological changes typical for apoptosis, such as chromatin condensation loss microvilli membrane. Addition NGF or rSCF prevented development characteristic decreased proportion apoptotic low content values dose-dependent manner. Polyclonal antibody completely abolished but not rSCF. NGF-induced G0/G1 phase cycle, transited them from S/G2M phase, suggesting NGF, unlike rSCF, may have no proliferation PMCs. By cytometric analysis antibodies receptors p75LNGFR p140trk, we defined expressed p140trk p75LNGFR. herbimycin A K-252a, tyrosine kinase inhibitors, resulted blockage phosphorylation restriction These results indicated suppressed through possessed proliferative activity. Thus, act key promote survival connective tissue-type cells.
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