Tissue-specific transcriptome characterization for developing tadpoles of the northern leopard frog (Lithobates pipiens)

摘要: A potential cause of amphibian population declines are the impacts environmental degradation on tadpole development. We conducted RNA sequencing developing northern leopard frog tadpoles and through de novo transcriptome assembly we annotated a large number open reading frames comparable in extent to genes identified Xenopus. Using our transcriptome, found transcript level changes between early (Gosner 26-31) late 36-41) stage were greatest tail, which is reabsorbed throughout There was an up-regulation immunity both head tail down-regulation associated with energy pathways mitochondria production myosin. Overall, across development consistent studies Xenopus findings highlight broader utility using RNA-seq identify differentially expressed response pressures.