Bone Marrow CD133+ Stem Cells Ameliorate Visual Dysfunction in Streptozotocin-induced Diabetic Mice with Early Diabetic Retinopathy.

摘要: Diabetic retinopathy (DR), one of the leading causes vision loss worldwide, is characterized by neurovascular disorders. Emerging evidence has demonstrated retinal neurodegeneration in early pathogenesis DR, and no treatment been developed to prevent neurodegenerative changes that precede detectable microvascular Bone marrow CD133+ stem cells with revascularization properties exhibit neuroregenerative potential. However, whether can ameliorate at stage DR remains unclear. In this study, mouse bone were immunomagnetically isolated analyzed for phenotypic characteristics, capacity neural differentiation, gene expression neurotrophic factors. After being labeled enhanced green fluorescent protein, intravitreally transplanted into streptozotocin (STZ)-induced diabetic mice assess outcomes visual function retina structure mechanism underlying therapeutic effect. We found co-expressed typical hematopoietic/endothelial stem/progenitor phenotypes, could differentiate lineage cells, expressed genes robust factors vitro. Functional analysis transplantation prevented dysfunction 56 days. Histological confirmed such a functional improvement showed survived, migrated inner (IR) over time preserved IR degeneration, including ganglion (RGCs) rod-on bipolar cells. addition, subset cell layer differentiated express RGC markers STZ-induced retina. Moreover, brain-derived (BDNFs) vivo increased BDNF level support survival Based on these findings, we suggest represents novel approach DR.