Characterization of a polyamine microsphere and its adsorption for protein.
作者： Feng Wang , Pei Liu , Tingting Nie , Huixian Wei , Zhenggang Cui
DOI: 10.3390/IJMS14010017
关键词:
摘要: A novel polyamine microsphere, prepared from the water-in-oil emulsion of polyethylenimine, was characterized. The investigation scanning electron microscopy showed that microsphere is a regular ball with smooth surface. diameter distribution 0.37–4.29 μm. isoelectric point 10.6. can adsorb proteins through co-effect electrostatic and hydrophobic interactions. Among tested, highest value adsorption 127.8 mg·g−1 obtained lipase. In comparison other proteins, force more important in promoting preferentially lipase an even mixture proteins. optimum temperature pH for selective by 35 °C 7.0.
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