Exposure to dsRNA Elicits RNA Interference in Brachionus manjavacas (Rotifera)

摘要: RNA interference (RNAi) is a powerful technique for functional genomics, yet no studies have reported its successful application to zooplankton. Many zooplankton, particularly microscopic metazoans of phylum Rotifera, unique life history traits which genetic investigation has been limited. In this paper, we report the development RNAi methods rotifers, with exogenous introduction double-stranded (dsRNA) through use lipofection reagent. Transfection dsRNA heat shock protein 90, membrane-associated progesterone receptor, and mitogen-activated kinase significantly increased proportion non-reproductive females. Additionally, fluorescence-based lectin binding assay confirmed significant suppression four six glycosylation enzymes that were targeted dsRNA. Suppression mRNA transcripts was quantitative PCR. Development rotifers promises enhance ability assessing regulation features critical their represents key step toward genomics research in