A liquid chromatographic-tandem mass spectrometric method for determination of artesunate and its metabolite dihydroartemisinin in human plasma

摘要: Background: Artemether–lumefantrine is the most widely recommended artemisinin-based combination treatment for falciparum malaria. Quantification of artemether and its metabolite dihydroartemisinin in biological matrices has traditionally been difficult, with sensitivity being an issue. Results: A high-throughput bioanalytical method analysis human plasma using solid-phase extraction 96-well plate format liquid chromatography coupled to positive ion mode tandem mass spectroscopy developed validated according US FDA guidelines. The uses 50 µl covers calibration range 1.43–500 ng/ml a limit detection at 0.36 ng/ml. Conclusions: chromatography–tandem spectrometry assay more sensitive than all previous methods despite lower volume (50 µl) highly suitable clinical studies where volumes are limited, such as pediatric trials.