Characterization of an immunofluorescence technique for the demonstration of coexisting neurotransmitters within nerve fibers and terminals.
作者： M W Wessendorf , R P Elde
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摘要: Neurotransmitters have been shown to coexist in cell bodies, but demonstrating their coexistence within nerve fibers and terminals has more difficult. However, two recent reports outlined a simple light-microscopic method by which neurotransmitters can be terminals. The was identical that used for immunohistochemical localization ofone antigen, except primary-secondary antibody systems labeled with different fluorochromes were simultaneously. In the present study, simultaneous visualization of serotonin substance P characterized. This employed an antiserum generated goat combination rabbitgenerated P. These antisera visualized secondary raised swine con
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