Granulocyte-Macrophage Colony Stimulating Factor Up-Regulates CCR1 in Human Neutrophils
作者： Sara S. Cheng , Joyce J. Lai , Nicholas W. Lukacs , Steven L. Kunkel
DOI: 10.4049/JIMMUNOL.166.2.1178
关键词:
摘要: Neutrophils (polymorphonuclear leukocytes; PMN) are phagocytic cells instrumental in the clearance of infectious pathogens. Human PMN commonly thought to respond primarily chemokines from CXC family. However, recent findings suggest that under specific cytokine activation conditions, can also some CC chemokines. In this study, effect GM-CSF, a well-characterized priming and maturation factor, on CC-chemokine receptor (CCR) expression was investigated. Constitutive CCR1 CCR3 mRNA detected by ribonuclease protection assay. Following incubation with GM-CSF (0.01–10 ng/ml; 6 h) rapidly (∼1 up-regulated. contrast, no significant induction CCR2, CCR3, CCR4, or CCR5 observed. protein up-regulated stimulation. GM-CSF-induced up-regulation showed functional consequences because GM-CSF-treated PMN, but not control cells, responded macrophage inflammatory protein-1α, monocyte chemoattractant protein-3, RANTES assays chemotactic migration intracellular calcium mobilization. These results activated proinflammatory change their pattern become responsive
highwire.org参考文章(57)
MA Brach, S deVos, HJ Gruss, F Herrmann, Prolongation of survival of human polymorphonuclear neutrophils by granulocyte-macrophage colony-stimulating factor is caused by inhibition of programmed cell death Blood. ,vol. 80, pp. 2920- 2924 ,(1992) , 10.1182/BLOOD.V80.11.2920.2920
Shaun R. McColl, Paul H. Naccache, Calcium mobilization assays. Methods in Enzymology. ,vol. 288, pp. 301- 309 ,(1997) , 10.1016/S0076-6879(97)88020-2
David Stephany, Ruth Swofford, Joshua M. Farber, Matthew K. Park, Fang Liao, Ronald L. Rabin, Chemokine Receptor Responses on T Cells Are Achieved Through Regulation of Both Receptor Expression and Signaling Journal of Immunology. ,vol. 162, pp. 3840- 3850 ,(1999)
T P Shanley, H P Friedl, P A Ward, M L Jones, H Schmal, Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats. Journal of Immunology. ,vol. 154, pp. 4793- 4802 ,(1995)
S. R. Mccoll, S. Levasseur, K. Neote, T. J. Schall, Mohamed Hachicha, Uncoupling of early signal transduction events from effector function in human peripheral blood neutrophils in response to recombinant macrophage inflammatory proteins-1 alpha and -1 beta. Journal of Immunology. ,vol. 150, pp. 4550- 4560 ,(1993)
M J Greenberger, T J Standiford, R G Kunkel, S L Kunkel, N W Lukacs, R M Strieter, J M Danforth, Macrophage inflammatory protein-1 alpha mediates lung leukocyte recruitment, lung capillary leak, and early mortality in murine endotoxemia. Journal of Immunology. ,vol. 155, pp. 1515- 1524 ,(1995)
J M Harter, R R Neubig, N Hassan, P J Mansfield, S J Suchard, G M Omann, A threshold level of coupled G-proteins is required to transduce neutrophil responses. Journal of Immunology. ,vol. 149, pp. 2172- 2178 ,(1992)
Philip M. Murphy, Shangming Zhang, Byoung S. Kwon, Byoung S. Kwon, Byung S. Youn, Ji Liang Gao, Differential Effects of Leukotactin-1 and Macrophage Inflammatory Protein-1α on Neutrophils Mediated by CCR1 Journal of Immunology. ,vol. 162, pp. 4938- 4942 ,(1999)
Marco Baggiolini, Chemokines and leukocyte traffic Nature. ,vol. 392, pp. 565- 568 ,(1998) , 10.1038/33340
Nadia Polentarutti, Raffaella Bonecchi, Walter Luini, Alessandro Borsatti, Charles Mackay, Alberto Mantovani, Alberto Mantovani, Christine Power, Amanda Proudfoot, Timothy N. C. Wells, Silvano Sozzani, Sergio Bernasconi, Massimo Locati, Up-regulation of CCR1 and CCR3 and induction of chemotaxis to CC chemokines by IFN-γ in human neutrophils Journal of Immunology. ,vol. 162, pp. 474- 479 ,(1999)