Structure and Characterization of the Human Tissue Inhibitor of Metalloproteinases-2 Gene

摘要: We report here the characterization of human tissue inhibitor metalloproteinases-2 (TIMP-2) gene. The gene is 83 kilobase pairs (kb) long with exon-intron splicing sites located in preserved positions among three members TIMP family. A 2.6-kb genomic DNA fragment flanking 5'-end contains several regulatory elements including five Sp1, two AP-2, one AP-1, and PEA-3 binding sites. Despite presence a complete AP-1 consensus at position -281, promoter did not respond to 12-O-tetradecanoylphorbol-13-acetate treatment. However, response was generated by insertion similar -71, indicating importance positioning this motif. typical CpG island; however, methylation island seem influence expression. Analysis 3'-end revealed that mRNAs for TIMP-2 (1.2 3.8 kb) differ selection their polyadenylation signal sites, but these does affect RNA stability. In summary, has features observed housekeeping genes, differs significantly from TIMP-1 TIMP-3 genes. These differences are likely explain specific roles inhibitors play regulation matrix metalloproteinases.