Acute phase proteins in rainbow trout (Oncorhynchus mykiss)

摘要: approved: Christopher J. Bayne The acute phase response (APR) includes an early of the innate immune system to inflammatory agent. Acute proteins (APP) are produced at increased rates in liver during this response. APR has been well characterized mammals, but it is unclear what degree teleosts mount a similar This thesis describes research conducted characterize rainbow trout. We further developed and used Tandem Crossed Immunoelectrophoresis Technique (TCIEP). With we were able determine that concentrations least three trout plasma higher several days after had injected with Freund's Complete Adjuvant. One these was isolated partially characterized, using electrospray mass spectrometry (ESI-MS), tentatively identified as transferrin. Due limited amount any protein could be obtained from T-CIEPs, switched use 2D gel electrophoresis based on isoelectric focusing SDS PAGE. When comparing such gels containing before treatment, 5 approximatly 200 spots size. An N-terminal sequence spots, degenerate primers constructed one those sequences; 5' 3' RACE PCR revealed 188 amino acid long polypeptide. It 29% identical level precerebellin rat. function unknown appears likely part since upregulated Redacted for Privacy Vibrio bacterin. begun characterization trout, much work remains conducted. ACUTE PHASE PROTEINS IN RAINBOW TROUT (Oncorhynchus inykiss).