Genome digestion is a dispensable consequence of physiological cell death mediated by cytotoxic T lymphocytes.

摘要: Abstract We examined virally transformed murine fibroblast clones as targets for cytotoxic T lymphocyte (CTL)-triggered lysis and genome digestion. Strikingly, while all were essentially equivalent in the ability to be lysed, one clone, SV3T3-B2.1, failed exhibit digestion associated with CTL attack. Other aspects of physiological cell death process, including loss adhesion nuclear envelope breakdown (lamin phosphorylation solubilization), not altered this clone. The absence CTL-induced correlated endodeoxyribonuclease activity nuclei that Characterization affected identifies a calcium-dependent, DNase I-like endonuclease approximately 40 kDa, normally present constitutively nuclei, enzyme responsible CTL-mediated death. These observations indicate neither per se nor its consequences [such activation poly(ADP-ribose) polymerase] are essential resulting from triggering suicide process.