Two-photon polymerization for production of human iPSC-derived retinal cell grafts.

摘要: Abstract Recent advances in induced pluripotent stem cell (iPSC) technology have paved the way for production of patient-specific neurons that are ideal autologous replacement treatment neurodegenerative diseases. In case retinal degeneration and associated photoreceptor therapy, polymer scaffolds critical cellular survival integration; however, prior attempts to materialize this concept been unsuccessful part due materials’ inability guide alignment. work, we used two-photon polymerization create 180 μm wide non-degradable prototype with varying pore sizes, slicing distances, hatching distances types. Hatching distance type were significant factors error vertical diameter, while most affected integrity geometry horizontal pores. We optimized printing parameters terms structural time order 1 mm loading studies. fabricated these larger structures directly on a porous membrane 3 µm diameter pores seeded them human iPSC-derived progenitor cells. After two days culture, cells nested extended neuronal processes parallel scaffolds, maximum occurring 25 μm These results highlight feasibility using technique as an strategy restoring vision patients degenerative Statement Significance Cell therapy is important goal investigators aiming restore neural function those suffering from disease. delivery frequently necessary success such treatments, but traditional biomaterials often fail facilitate orientation close packing needed recapitulate vivo environment. Here, use densely packed small, interconnected nutrient diffusion. This study offers thorough characterization how affect final outcomes time. Our findings demonstrate can align 3D large enough be transplantation. future could comprise biodegradable materials tunable microstructure, elastic modulus degradation time; step towards promising option late-stage neurodegeneration, including blindness.