Immunomodulatory effects ofBacillus subtilis(natto) B4 spores on murine macrophages

摘要: To investigate the immunomodulatory effects of Bacillus subtilis (B. subtilis) (natto) B4 spores on murine macrophage, RAW 264.7 cells were cultured alone or with B at 37°C for 12 hrs, then both and culture supernatants collected analyses. Exposure to B. had no significant macrophage viability amounts extracellular lactate dehydrogenase (LDH). However, it remarkably increased activities acid phosphatase (ACP), (LDH) inducible nitric oxide synthase (iNOS) in (NO) cytokines (tumor necrosis factor-alpha, interferon-gamma, interleukin [IL]-1 beta, IL-6, IL-12, IL-10 inflammatory protein-2) supernatants. These results demonstrate that are harmless macrophages can stimulate their activation through up-regulation ACP LDH enhance immune function by increasing iNOS activity stimulating NO cytokine production. The above findings suggest have macrophages.