Thioredoxin and thioredoxin reductase influence estrogen receptor α-mediated gene expression in human breast cancer cells
作者： Abhi K Rao , Yvonne S Ziegler , Ian X McLeod , John R Yates , Ann M Nardulli
DOI: 10.1677/JME-09-0053
关键词:
摘要: Accumulation of reactive oxygen species (ROS) in cells damages resident proteins, lipids, and DNA. In order to overcome the oxidative stress that occurs with ROS accumulation, must balance free radical production an increase level antioxidant enzymes convert radicals less harmful species. We identified two enzymes, thioredoxin (Trx) Trx reductase (TrxR), a complex associated DNA-bound estrogen receptor (ERa). Western analysis immunocytochemistry were used demonstrate TrxR are expressed cytoplasm nuclei MCF-7 human breast cancer cells. More importantly, endogenously ERa, Trx, interact ERa associate native, estrogen-responsive pS2 progesterone genes RNA interference assays demonstrated differentially influence gene expression together, 17b-estradiol, alter hydrogen peroxide (H2O2) levels Our findings suggest multifunctional proteins that, addition modulating H2O2 transcription factor activity, aid regulating target
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