作者: Daibin Zhong , Cristian Koepfli , Liwang Cui , Guiyun Yan
DOI: 10.1186/S12936-018-2322-5
关键词:
摘要: Multiplicity of infection (MOI), also termed complexity (COI), is defined as the number genetically distinct parasite strains co-infecting a single host, which an important indicator malaria epidemiology. PCR-based genotyping often underestimates MOI. Next generation sequencing technologies provide much more accurate and genome-wide characterization polyclonal infections. However, complete haplotype multiclonal infections remains challenge due to PCR artifacts errors, requires efficient computational tools. In this review, advantages limitations current molecular approaches determine multiplicity are discussed.