Development and Evaluation of Novel Real-Time Reverse Transcription-PCR Assays with Locked Nucleic Acid Probes Targeting Leader Sequences of Human-Pathogenic Coronaviruses
作者： Jasper Fuk-Woo Chan , Garnet Kwan-Yue Choi , Alan Ka-Lun Tsang , Kah-Meng Tee , Ho-Yin Lam
DOI: 10.1128/JCM.01224-15
关键词:
摘要: Based on findings in small RNA-sequencing (Seq) data analysis, we developed highly sensitive and specific real-time reverse transcription (RT)-PCR assays with locked nucleic acid probes targeting the abundantly expressed leader sequences of Middle East respiratory syndrome coronavirus (MERS-CoV) other human coronaviruses. Analytical clinical evaluations showed their noninferiority to a commercial multiplex PCR test for detection these
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