Production and characterization of low molecular weight heparosan in Bacillus megaterium using Escherichia coli K5 glycosyltransferases.

摘要: Abstract Low molecular weight heparosan is an un-sulfated polysaccharide primarily used as a precursor for heparin synthesis that has recently been in drug delivery applications. Heparosan from recombinant bacterial systems provides safer alternative to naturally producing pathogenic systems. In this study, we engineered functional pathway Bacillus megaterium by the expression of E. coli K5 kfiC and kfiA glycosyltransferase genes. Upregulation individual UDP-sugar genes enhanced production, indicating UDP-precursor sugar concentrations were limiting biosynthesis. The B. yielded maximum concentration 394 mg/L batch bioreactor. titer was further increased 1.32 g/L fed-batch fermentation. Nuclear magnetic resonance analysis revealed chemical structure derived identical heparosan. varied 31 60 kDa, its potential chemoenzymatic This study efficient process produce non-pathogenic megaterium.