miR-17 enhances proliferation and migration and inhibits apoptosis in glioma cells by regulating SASH1 expression
作者： Qi Yao , Yaohua Yan , Jianguo Chen , Haitao Zhang , Liu Yang
DOI: 10.21037/7261
关键词:
摘要: Background: To investigate the mechanisms underlying regulation of human glioma cell growth, proliferation, and apoptosis by microRNA-17 (miR-17) to provide targets for novel therapies. Methods: The expression miR-17 in tissues lines was detected using real-time quantitative PCR (qRT-PCR). A inhibitor transfected into U251 line liposomes, changes were qRT-PCR. effects on numerous biological characteristics cells, including viability, apoptosis, migration, analyzed MTT, flow cytometry, Transwell migration chamber assays. luciferase reporter gene system used validate SAM- SH3-domain containing 1 ( SASH1 ) as a true target miR-17. protein determined western blotting. Results: qRT-PCR results showed that compared with adjacent tissues, significantly increased cells (P Conclusions: promotes inhibits these could be mediated expression.
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