Clock represses preadipocytes adipogenesis via GILZ.

摘要: Adiposity is a worldwide health threat that needs to be prevented. Circadian gene Clock (circadian locomotor output cycles kaput) closely correlated adiposity; for example, weight gain, adipocytes size expansion, and serum lipid level rise in ClockΔ19 mice compared C57BL/6J mice. However, the precise role of during adipogenic differentiation unknown. Herein, circadian shown regulate adipogenesis mediated by GILZ. Clock-mediated attenuation upregulation influenced synthesis affected levels transcriptional factors, C/EBP-β, C/EBP-α, PPAR-γ, FABP4, both vivo vitro (primary adipose-derived stromal cells 3T3-L1 cells). Chromatin immunoprecipitation (ChIP) assay, reporter shock assay found transcriptionally regulated glucocorticoid-induced leucine zipper (GILZ). Furthermore, GILZ could relieve inhibitory effect on overexpression also reduced promotion suggesting inhibits preadipocytes via The current results demonstrate how genes are likely adiposity, affecting process, as well as, increasing fat number. Therefore, this study may provide novel insights into underlying mechanism explaining correlation between mutation adiposity.