Rapid Screen of Potential i-Motif Forming Sequences in DNA Repair Gene Promoters

摘要: We have studied the in vitro stability of 25 potential i-motif-forming DNA sequences found within promoter regions 18 different human repair genes. Three widely available methods characterization were used to rapidly assess i-motif folding and comprise a simple screen for preliminary identification physiologically relevant forming sequences. Four highly pH-stable candidate identified exhibiting pH transitions (pH at which 50% oligodeoxynucleotides solution are folded) or above 6.6, thermal melting temperatures 37 °C isothermal UV difference spectra characteristic 2'-deoxycytidine imino-nitrogen protonation. These newly could represent novel targets understanding modulating gene expression.