Niflumic and flufenamic acids are potent reversible blockers of Ca2(+)-activated Cl- channels in Xenopus oocytes.

摘要: The effects of niflumic acid and flufenamic acid, two nonsteroidal anti-inflammatory agents known to block anion transport in red blood cells, on Ca2(+)-activated Cl- currents (ICl(Ca)) Xenopus oocytes were examined. Both compounds reversibly inhibited ICl(Ca), elicited response depolarizing voltage steps, a dose-dependent manner, with no effect the shape current-voltage curve. apparent inhibition constant for was 17 microM, whereas that 28 microM. Niflumic also ICl(Ca) by bath application Ca2+ permeabilized using ionophore A23187, demonstrating is due direct interaction channel, rather than interference entry through voltage-dependent channels. In addition their use elimination as possible source artifact when are used an expression system exogenous ion channels receptors, it expected these will find potent channel blockers.