Mass spectrometry imaging of glycans from tissue sections and improved analyte detection methods

摘要: The presently disclosed subject matter provides methods using mass spectrometry for direct profiling of N-linked glycans from a biological sample. In addition, the embodiments present invention also disclose novel methods, known as targeted analyte detection (TAD), improving limit MALDI-MS. These take advantage carrier effect added standard analytes, which occurs due to generic sigmoidal shape calibration curve. functionality TAD depends on relative enhancement sensitivity over increase deviation at analysis target analytes with spiking in exogenous concentration. At certain ranges concentration, increment overcomes deviation, resulting an improved LOD. Theoretically, concentrations approximately 1 LODorig would generate optimum LOD improvement. is cost-effective improvement method, not limited group or instruments. It can be applied enhance any different provided that interest extracted available synthetic form.