Enzymatic conversion of sterigmatocystin into aflatoxin B1 by cell-free extracts of Aspergillus parasiticus.

摘要: A cell-free extract, prepared from Aspergillus parasiticus ATCC 15517 grown in synthetic medium, was active converting [14C]sterigmatocystin into aflatoxin B1 the presence of reduced nicotinamide adenine dinucleotide phosphate. The activity demonstrated by time course conversion and linear dependence yield product on enzyme concentrations. Optimum obtained at pH 7.5 to 7.8 27 C. results confirm sterigmatocystin as a biogenetic precursor B1. Techniques were developed for enzymatic studies biosynthesis.