Enlarged and highly repetitive plastome of Lagarostrobos and plastid phylogenomics of Podocarpaceae

作者: Edi Sudianto , Chung-Shien Wu , Lars Leonhard , William F. Martin , Shu-Miaw Chaw

DOI: 10.1016/J.YMPEV.2018.12.012

关键词:

摘要: Podocarpaceae is the largest family in cupressophytes (conifers II), but its plastid genomes (plastomes) are poorly studied, with plastome data currently existing for only four of 19 Podocarpaceous genera. In this study, we sequenced and assembled complete plastomes from representatives eight additional genera, including Afrocarpus, Dacrydium, Lagarostrobos, Lepidothamnus, Pherosphaera, Phyllocladus, Prumnopitys, Saxegothaea. We found that a monotypic genus native to Tasmania, has (151,496 bp) among any studied date. Plastome enlargement Lagarostrobos coincides increased intergenic spacers, repeats, duplicated genes. Among Podocarpaceae, most rearranged plastome, substitution rates modest. Plastid phylogenomic analyses based on 81 genes clarify positions previously conflicting Tree topologies firmly support division into two sister clades: (1) Prumnopityoid clade (2) containing Podocarpoid, Dacrydioid, The Phyllocladus nested within thus familial status Phyllocladaceae not supported.

参考文章(73)
Robert K. Jansen, Tracey A. Ruhlman, Plastid Genomes of Seed Plants Advances in Photosynthesis and Respiration. pp. 103- 126 ,(2012) , 10.1007/978-94-007-2920-9_5
CN, Jr Stewart, Laura E Via, A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. BioTechniques. ,vol. 14, pp. 748- 750 ,(1993)
Steven J. Wagstaff, Evolution and biogeography of the austral genus Phyllocladus (Podocarpaceae) Journal of Biogeography. ,vol. 31, pp. 1569- 1577 ,(2004) , 10.1111/J.1365-2699.2004.01066.X
Diego Darriba, Guillermo L Taboada, Ramón Doallo, David Posada, jModelTest 2: more models, new heuristics and parallel computing Nature Methods. ,vol. 9, pp. 772- 772 ,(2012) , 10.1038/NMETH.2109
W.T. Sinclair, R.R. Mill, M.F. Gardner, P. Woltz, T. Jaffré, J. Preston, M.L. Hollingsworth, A. Ponge, M. Möller, Evolutionary relationships of the New Caledonian heterotrophic conifer, Parasitaxus usta (Podocarpaceae), inferred from chloroplast trn L-F intron/spacer and nuclear rDNA ITS2 sequences Plant Systematics and Evolution. ,vol. 233, pp. 79- 104 ,(2002) , 10.1007/S00606-002-0199-8
Andrew J. Alverson, Shi Zhuo, Danny W. Rice, Daniel B. Sloan, Jeffrey D. Palmer, The Mitochondrial Genome of the Legume Vigna radiata and the Analysis of Recombination across Short Mitochondrial Repeats PLoS ONE. ,vol. 6, pp. e16404- ,(2011) , 10.1371/JOURNAL.PONE.0016404
Koichiro Tamura, Fabia Ursula Battistuzzi, Paul Billing-Ross, Oscar Murillo, Alan Filipski, Sudhir Kumar, None, Estimating divergence times in large molecular phylogenies Proceedings of the National Academy of Sciences of the United States of America. ,vol. 109, pp. 19333- 19338 ,(2012) , 10.1073/PNAS.1213199109