Stereochemical control of the dna binding affinity, sequence specificity, and orientation-preference of chiral hairpin polyamides in the minor groove

摘要: This invention provides improved polyamides comprising a hairpin loop derived from .gamma.-aminobutyric acid which bind to the minor groove of promoter regions DNA sequence. Binding polyamide sequence region inhibits expression requisite gene. The improvement relates use R-2,4-diaminobutyric and derivatives 2-amino group form loop. asymmetric for tighter binding additionally an amine function derivatizing by, example, forming amide linkages. Such may serve attach detectable labels polyamide.