Epithelial Growth Factor-Anchored on Polycaprolactone/6-deoxy-6-amino- β -cyclodextrin Nanofibers: In Vitro and In Vivo Evaluation.

摘要: Polycaprolactone (PCL) is a well-known FDA approved biomaterial for tissue engineering. However, its hydrophobic properties limit use skin wound healing which makes functionalization necessary. In this work, we present the fabrication and evaluation of PCL nanofibers by electrospinning technique, as well functionalized with 6-deoxy-6-amino-β-cyclodextrin (aminated nanofibers). Afterwards, epithelial growth factor (EGF) was anchored onto hydrophilic PCL/deoxy-6-amino-β-cyclodextrin. The characterization three electrospun fibers made means field emission scanning electron microscopy (FESEM), Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR); Confocal-Raman Spectroscopy were used elucidated chemical structure, hydrophilicity determined Contact Angle (CA). vitro cell proliferation test seeding embryonic fibroblast line (3T3) mats in vivo studies murine model conducted to prove effectivity material. showed that aminated without EGF had 100 150% more 3T3 cells against alone, respectively. results accelerated incorporation EGF. addition, favorable effects epidermal proliferation. study demonstrates protein high biological interest like can be attached covalently surface synthetic material enriched amino groups. This kind has great potential applications regeneration healing.