On-chip ultraviolet holography for high-throughput nanoparticle and biomolecule detection

摘要: Nanoparticle and biomolecule imaging has become an important need for various applications. In effort to find a higher throughput alternative existing devices, we have designed lensfree on-chip holographic platform operating at ultraviolet (UV) wavelength of 266 nm. With custom-designed free-space light delivery system illuminate the sample that is placed very close ( 16 mm 2 ) was utilized as field-of-view (FOV) capturing signatures target objects on chip. These holograms were then digitally back propagated extract both amplitude phase information sample. The increased forward scattering from nanoparticles due this shorter illumination enabled us image individual particles are smaller than 30 nm over FOV >16 . Our further in high-contrast nanoscopic aggregates since strongly absorbed by biomolecules including proteins nucleic acids. Aggregates Cu/Zn-superoxide dismutase (SOD1), which been linked fatal neurodegenerative disease, ALS (amyotrophic lateral sclerosis), imaged with significantly improved contrast compared visible wavelengths. This unique UV modality could be valuable biomedical applications (e.g., viral load measurements) environmental monitoring air water quality monitoring.