Differential expression of genes during the interaction between Colletotrichum lindemuthianum and Phaseolus vulgaris

摘要: The fungus Colletotrichum lindemuthianum is the causal agent of anthracnose, one most severe diseases common bean (Phaseolus vulgaris). infection process begins with adhesion conidia to plant’s surface. Appressoria are then formed, allowing penetration fungus. Next, biotrophic phase begins, followed by necrotrophic phase. Due peculiar nutrition mode fungus, including both previously mentioned stages, it great interest determine which genes involved in transition between two phases during process. To this, suppression subtractive hybridization (SSH) was used association qRT-PCR present study. These methods allowed for identification that were differentially expressed at each developmental stage plant. This first report on use cited techniques evaluate infectious cycle A total 175 sequences exhibited significant identity (e ≤ 10−5) sequenced genomes P. vulgaris and C. lindemuthianum; approximately 41 % those determined belong 59 % Of predicted sequences, 68 % unknown function or not found databases. Among analyzed sequence tags (ESTs), encode proteins related to: primary secondary metabolism; transport different compounds; degradation/modification proteins; cell regulation signaling; cellular stress response; degradation exogenous compounds. obtained results encoding essential progression anthracnose. Furthermore, possible identify new genes, functions have yet been described, even unique pathogenicity virulence this