Direct visualization of an antidepressant analog using surface-enhanced Raman scattering in the brain.

摘要: Detailed spatial information of low-molecular weight compound distribution, especially in the brain, is crucial to understanding their mechanism actions. Imaging techniques that can directly visualize drugs brain at a high resolution will complement existing tools for drug distribution analysis. Here, we performed surface-enhanced Raman scattering (SERS) imaging using bioorthogonal alkyne tag situ resolution. Focusing on selective serotonin reuptake inhibitor S-citalopram (S-Cit), which possesses nitrile group, substituted an alkynyl group into its structure and synthesized alkynylated S-Cit (Alk-S-Cit). The transitivity inhibition Alk-S-Cit were not significantly different as compared with S-Cit. was visualized coronal mouse section SERS silver nanoparticles. Furthermore, combined fluorescence microscopy allowed be adjacent neuronal membranes, well vessel parenchyma. Therefore, our multimodal technique effective method detecting compounds original tissue environment potentially offer additional regarding precise such drugs.