Accumulation of the D2 Protein Is a Key Regulatory Step for Assembly of the Photosystem II Reaction Center Complex in Synechocystis PCC 6803

摘要: Accumulation of monomer and dimer photosystem (PS) II reaction center core complexes has been analyzed by two-dimensional Blue-native/SDS-PAGE in Synechocystis PCC 6803 wild type mutant strains lacking genes psbA, psbB, psbC, psbDIC/DII, or the psbEFLJ operon. In vivo pulse-chase radiolabeling experiments revealed that cells assembled PSII precomplexes only. DeltapsbC DeltapsbB, assembly cores CP43 was detected, respectively. DeltapsbA, protein subunits CP43, CP47, D2, cytochrome b559 were synthesized, but proteins did not assemble. Similarly, DeltapsbD/C de novo synthesized D1, form any mutual complexes, indicating complex is a prerequisite for with CP47 CP43. Finally, although accumulated DeltapsbEFLJ, D2 neither expressed nor accumulated. We, furthermore, show amount high strain whereas D1 low D2. We conclude expression operon accumulation key regulatory step consecutive complex.