Mineralization of the s-triazine ring of atrazine by stable bacterial mixed cultures.

摘要: Enrichment cultures containing atrazine (2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine) at a concentration of 100 ppm (0.46 mM) as sole nitrogen source were obtained from soils exposed to repeated spills atrazine, alachlor, and metolachlor. Bacterial growth occurred concomitantly with formation metabolites subsequent biosynthesis protein. When ring-labeled [14C]atrazine was used, 80% or more the s-triazine ring carbon atoms liberated 14CO2. Hydroxyatrazine may be an intermediate in mineralization pathway. More than 200 pure isolated enrichment failed utilize source. Mixing restored atrazine-mineralizing activity. Repeated transfer mixed led increased rates metabolism. The rate degradation, even elevated concentrations far exceeded previously reported soils, waters, bacteria.