Metabolite profiling for plant functional genomics
作者： Oliver Fiehn , Joachim Kopka , Peter Dörmann , Thomas Altmann , Richard N. Trethewey
DOI: 10.1038/81137
关键词:
摘要: Multiparallel analyses of mRNA and proteins are central to today's functional genomics initiatives. We describe here the use of metabolite profiling as a new tool for a comparative display of gene function. It has the potential not only to provide deeper insight into complex regulatory processes but also to determine phenotype directly. Using gas chromatography/mass spectrometry (GC/MS), we automatically quantified 326 distinct compounds from Arabidopsis thaliana leaf extracts. It was possible to assign a chemical …
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